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scpA [2018-08-15 10:48:00]
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scpA [2018-08-15 10:48:00]

part of the [SW|condensin] complex, chromosomal origin condensation and segregation
locus
BSU23220
pI
4.79
mw
29.46 kDa
protein length
251 aa Sequence Blast
gene length
753 bp Sequence Blast
function
segregation of replication origins
product
DNA segregation and condensation protein
essential
yes
synonyms
ypuG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    2,425,831 → 2,426,586

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • ''[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]

    • The protein

    Protein family

  • scpA family (according to Swiss-Prot)

    • Structure

  • [PDB|3ZGX] (complex between the head domain of [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]) [Pubmed|23353789]
  • [PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] (aa 1-183) [Pubmed|23353789]

    • [SW|Localization]

  • nucleoid (Multiple) [Pubmed|16479537]
  • the [SW|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|ParA] [Pubmed|24440393,23475963]*the [SW|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB] to ''parS'' centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]

    • Expression and Regulation

    Operons

    genes
    [gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]-[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]
    description
    [Pubmed|7934830]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
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    Biological materials

    Mutant

  • MGNA-A104 (ypuG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/104 NBRP B. subtilis, Japan]

    • References

    Reviews

  • 22934648,22933559,24118085,25460803,26706151,29182912

    • Original publications

  • 12100548,12421306,12065423,7934830,16479537,19450516,15009890,11948165,23353789,23475963,22385855,24440399,24440393,25557547,26253537,25951515,26295962,26725510,26904953,28154080,28286005,28689660